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. 2022 Apr 21;14(5):295. doi: 10.3390/toxins14050295

Figure 3.

Figure 3

Chromatographic profile of P. depilata’s venom by RP-HPLC. The venom was separated using a C18 analytical column (250 × 4.6 mm), with an elution gradient from 0 to 60% ACN plus 0.1% TFA, for 60 min, with a flow of 1 mL/min. The absorbance was monitored at 230 nm. The percentages shown on each fraction correspond to the ACN gradient of elution.