Figure 3.

Temperature influences on mosquito competence for MAYV. Mosquitoes were challenged with ingestion of MAYV-infected bloodmeal (7 log₁₀ PFUe/mL) for 1 h at 28 °C; then, engorged females were placed in separate environmental incubators (20 °C = low; 30 °C = high) for the remainder of the study. At 3, 9, 15, 21, and 27 dpi, mosquitoes (n = 30/time point) were anesthetized with CO₂, and their wings and legs were removed from their bodies, followed by a collection of salivary secretions. (A) MAYV susceptibility to infection (body), (C) disseminated infection (legs), and (E) transmission (salivary secretions) expressed in percentages. The kinetics of MAYV growth in mosquito tissues and salivary secretions were determined by qRT-PCR. (B) MAYV titer of body, (D) legs, and (F) salivary secretions, represented as (PFUe/mL). Each data point in (B,D,F) represents the kinetics of MAYV growth (titer) of individual mosquitoes. Gray data points in (B) represent mosquitoes with non-disseminated infection (i.e., MAYV infection limited to the midgut). Numbers in brackets above bars indicate the total number of mosquitoes positive for MAYV. Horizontal lines indicate the mean ± SEM. Statistical significance was determined using logistic regression analysis and two-way ANOVA for MAYV infection measurements and titers, respectively. p-values were corrected for multiple comparisons. Asterisks (*) denote significant differences; p < 0.05.