Figure 2.
BcWCL1 and BcWCL2 proteins interact in the presence of blue light or its absence. Luciferase expression was measured as luminescence in arbitrary units (a.u.) of the yeast cell cultures. The protein–protein interaction activates luciferase expression controlled by the 5XGAL1 synthetic promoter (P5XGAL1) under three different experimental conditions: (A) constant darkness (DD), (B) constant blue light (BL), and (C) a single blue-light pulse (BLP) of 2 h duration (between dotted lines). In all panels, the FUN-LOV optogenetic switch [32] was used as a positive control of light-mediated protein–protein interaction that activates Luc expression. The average of luciferase expression determined in six biological replicates is shown, with the standard deviation represented as a shaded grey region.