Table 2.

Some key considerations for maintaining consistent quality of mosquitoes being reared for bioassays and conditions during bioassays, and steps that can be taken to monitor quality of mosquitoes.

Parameters to Standardise When Rearing Mosquitoes	Suggested Quality Control Processes in Mosquito Rearing	Parameters to Standardise When Performing Bioassays
Temperature Relative humidity (RH) Controlled light/dark cycle 1 h ‘dawn’ and ‘dusk’ Larval density and feeding regime Adult density in cages Non-limiting access to a sugar solution	Follow detailed rearing SOP Routine monitoring of some fitness indicator/s 1 to follow colony health and rearing quality Regular selection with at least one insecticide Periodic profiling of resistance phenotype Maintain staff training records on SOPs covering rearing and quality control Equipment maintenance and calibration Keep a record of deviations from SOP	Temperature Relative humidity (RH) Time of day 2 Feeding status (sugar, water, blood) Age of mosquito Measure a fitness indicator in testing cohort Maintain staff training records on SOPs covering testing, data handling, archiving etc.

¹ In decreasing order of preference): composite fitness indices, wing morphometrics [69], wing length, dry weight, wet weight; ² Mosquitoes may be reared on an adjusted light cycle to accommodate testing at a specific point in their circadian rhythm within working hours.