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. 2022 May 20;23(10):5752. doi: 10.3390/ijms23105752

Figure 2.

Figure 2

N3 peptide binds to TREM-1. (A) Elution of biotin-N3 peptide from sTREM-1 conjugated with CNBr-activated sepharose. 1—washing before elution, 2—elution of N3 peptide, 3—control N3 peptide. (B,C) Crosslinking N3 peptide with TREM-1 on monocytes surface. Binding N3 peptide with TREM-1 on monocytes surface in presence of BS3. Cells were lysed and purified with anti-TREM-1 Dynabeads (B) or anti-Tag7 Dynabeads (C). Bound material was analyzed by SDS-PAGE followed by WB and detected with anti-Tag7 (B) and anti-TREM-1 (C) respectively.