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. 2022 May 21;12(5):841. doi: 10.3390/jpm12050841

Figure 2.

Figure 2

MRE11 regulates CD44 expression and tumorsphere formation in OSCC cells. (A) Total protein lysates from CAL-27 cells carrying overexpression vector of MRE11 (MRE11-OE) or empty vector (EV) and from HSC-3 cells carrying shRNA knockdown vector targeting MRE11 (shMRE11) or shRNA vector targeting firefly luciferase (shLuc) were collected and analyzed by Western blot. (B,C) CAL-27 cells carrying overexpression vector of MRE11 (MRE11-OE) or empty vector (EV) in (B) and HSC-3 cells carrying shRNA knockdown vector targeting MRE11 (shMRE11) or shRNA vector targeting firefly luciferase (shLuc) in (C) were collected and re-plated in ultra-low attachment microplates for tumorsphere formation assay. Quantitation of tumorspheres was carried out for those with diameter > 50 μm. Data were obtained from three independent experiments and presented as mean ± SEM. Statistical difference was determined by Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.