TABLE 3.

EPS production by supernatants and washed cells of L. reuteri LB 121 and mutant strain 35-5 incubated at 37°C in buffer with sucrose as the substrate^a

Sugar in growth medium	Washed cells or supernatant	Incubation time (h)	Glucan produced by strain 35-5 (g · liter−1)	Glucan produced by strain LB 121 (g · liter−1)	Fructan produced by strain LB 121 (g · liter−1)
Sucrose	Washed cells	0	0.28	ND	ND
		2	0.60	0.43	0.41
		10	3.12	1.19	0.92
		22	4.52	1.87	1.57
Sucrose	Supernatant	0	0.36	ND	ND
		2	0.44	0.37	0.55
		10	0.52	0.64	1.06
		22	1.00	0.85	1.70
Glucose	Washed cells	0	0.32	ND	ND
		2	1.24	0.89	0.27
		10	3.76	3.01	0.97
		22	5.72	4.35	1.51
Glucose	Supernatant	0	0.16	ND	ND
		2	0.08	ND	ND
		10	0.04	ND	ND
		22	0.12	ND	ND

^aCultures were harvested in the exponential phase of growth and incubated with sucrose plus 50 μg of chloramphenicol to block de novo enzyme synthesis. ND, not detected.