Table 1.

SPR kinetics results for unmodified and modified protein–polymer conjugates against β-CD immobilized CM5 chip, diH₂O running buffer.

Interaction (Host/Ligand)	KD (M)	Binding Free Energy (KJ/mol)
βCD/BSA	0.001293 1	−16.5
βCD/BSA–(PEG5000–Ad)2	7.966 × 10−5 1	−23.4
βCD/BSA–(PEG5000–Ad)4	9.090 × 10−9	−45.9
βCD/BSA–(PEG5000–Ad)8	4.099 × 10−10	−53.6
βCD/mAb	0.0001443 1	−21.9
βCD/mAb–(PEG5000–Ad)2	8.755 × 10−10 1	−52.9
βCD/mAb–(PEG5000–Ad)4	3.012 × 10−11	−54.1
βCD/mAb–(PEG5000–Ad)8	4.515 × 10−13	−70.4

¹ K_D values were within the model confidence interval with Chi² values below 10% of the maximum SPR response.