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. 2022 May 23;11(10):1382. doi: 10.3390/plants11101382

Figure 2.

Figure 2

Subcellular localization and dimer-forming ability of ZmLBD5: (A–C) Subcellular localization of ZmLBD5 in tobacco leaves, maize protoplasts, and 35S::ZmLBD5-eGFP transgenic Arabidopsis, respectively (bar = 50, 10, and 20 μm, respectively). (D) Three fragments of ZmLBD5 (A–C) were divided and the red triangle indicated the position of truncation. (E) The ability of ZmLBD5 to form dimers in the yeast strain Y2H Gold. The Y2H Gold strains containing target plasmids were diluted and cultured on no-selection synthetic dropout (SD) media without tryptophan and leucine (SD/-T-L), or on selection SD media without tryptophan, leucine, histidine, and adenine (SD/-T-L-H-A). Photos were taken 3 days after inoculation for the plates. Fragments A, B, and C represent CX2CX6CX3C, GAS, and LX6LX3LX6L, and various C-terminal domains, respectively.