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. 2022 May 23;11(10):1382. doi: 10.3390/plants11101382

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression levels of drought-stress-related genes in ZmLBD5 transgenic Arabidopsis: Total RNA was isolated from 15-day-old seedlings grown without (CK) or with 250 mM mannitol treatment for 7 days. Transcript levels of PP2CA, RD17, RD26, DREB2A, RD29A, and RD29B in the transgenic lines and wild type were determined by qPCR, using AtACTIN8 and AtUBQ10 as reference genes. Fold change was calculated by 2^−∆∆t. Significance was analyzed by one-way ANOVA. * p < 0.05; ** p < 0.01; *** p < 0.001. All bars represent means ± SD (n = 3).