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. 2022 Apr 26;20(5):291. doi: 10.3390/md20050291

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic tree of the Kunitz venom protein family. The maximum likelihood (ML) tree was constructed based on amino acid sequences using IQ-TREE version 2.1.1 software. The numbers below the nodes show bootstrap support values from 1000 replicates. ML phylogram was generated from amino-acid alignments of Kunitz homologs using the auto model. The location and length of conserved domains for each amino acid are shown on the right. Putative sequences outlined in pink, purple, and grey are from myxozoans, free-living cnidarians, and other toxic organisms.