Figure 9.

Scheme of nimodipine and cisplatin treatment. To investigate the protective effect of nimodipine on cisplatin-associated ototoxicity UB/OC−1 and UB/OC−2 were pre-treated with different concentrations of nimodipine for 24 h (a). Afterward, stress was induced by 20 µM, 50 µM, and 100 µM cisplatin while the same amount of nimodipine was added again. Experiments were performed 24 h after the cell stress was triggered (b). Microscopic images of UB/OC−1 and UB/OC−2 cells were created with Keyence BZ-X810 (Keyence, Neu-Isenburg, Germany).