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. 2022 May 21;23(10):5780. doi: 10.3390/ijms23105780

Table 2.

Antibodies used for Western Blot.

Antibody Isotype Dilution Dilution Buffer Manufacturer
Phospho-Akt (Ser473) (D9E) XP® #4060 Rabbit IgG 1:1000 5% BSA in TBS-T Cell Signaling Technology (Danvers, MA, USA)
Akt (pan) (40D4) #2920 Mouse IgG1 1:2000 5% MP in TBS-T
Phospho-CREB (Ser133) (87G3) #9198 Rabbit IgG 1:1000 5% MP in TBS-T
CREB (48H2) #9197 Rabbit IgG 1:1000 5% BSA in TBS-T
Phospho-Stat3 (Tyr705) (3E2) #9138 Mouse IgG1 1:1000 5% MP in TBS-T
Stat3 (124H6) #9139 Mouse IgG2a 1:1000 5% MP in TBS-T
LMO4 (D6V4Z) #81428 Rabbit IgG 1:1000 5% BSA in TBS-T
GAPDH (14C10) #2118 Rabbit IgG 1:1000 5% BSA in TBS-T
Anti-mouse IgG, HRP-linked Antibody Horse 1:1000 2% MP in TBS-T
Anti-rabbit IgG, HRP-linked Antibody Goat 1:1000 2% MP in TBS-T

BSA, bovine serum albumin (Carl Roth, Karlsruhe, Germany); GAPDH, glyceraldehyde-3-phosphatedehydrogenase; MP, skim milk powder; IgG, immunoglobulin G.