Table 2.
Antibody | Isotype | Dilution | Dilution Buffer | Manufacturer |
---|---|---|---|---|
Phospho-Akt (Ser473) (D9E) XP® #4060 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | Cell Signaling Technology (Danvers, MA, USA) |
Akt (pan) (40D4) #2920 | Mouse IgG1 | 1:2000 | 5% MP in TBS-T | |
Phospho-CREB (Ser133) (87G3) #9198 | Rabbit IgG | 1:1000 | 5% MP in TBS-T | |
CREB (48H2) #9197 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | |
Phospho-Stat3 (Tyr705) (3E2) #9138 | Mouse IgG1 | 1:1000 | 5% MP in TBS-T | |
Stat3 (124H6) #9139 | Mouse IgG2a | 1:1000 | 5% MP in TBS-T | |
LMO4 (D6V4Z) #81428 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | |
GAPDH (14C10) #2118 | Rabbit IgG | 1:1000 | 5% BSA in TBS-T | |
Anti-mouse IgG, HRP-linked Antibody | Horse | 1:1000 | 2% MP in TBS-T | |
Anti-rabbit IgG, HRP-linked Antibody | Goat | 1:1000 | 2% MP in TBS-T |
BSA, bovine serum albumin (Carl Roth, Karlsruhe, Germany); GAPDH, glyceraldehyde-3-phosphatedehydrogenase; MP, skim milk powder; IgG, immunoglobulin G.