Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Jul;65(7):3021–3026. doi: 10.1128/aem.65.7.3021-3026.1999

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 1999, American Society for Microbiology

PMC Copyright notice

FIG. 1 — Construction of the recombinant B. sphaericus strains by homologous recombination. The genes were introduced either upstream from the binary toxin (cry11A) or at the protease locus (cry11A, cry11Ba, and aphA3). E1, EcoRI. Probes used in Southern blot analysis were the 5′ end of the binary toxin (BamHI/BglII fragment from pHT560) (), the 5′ end of the protease gene (BamHI/SalI fragment from pPS408) (▪), the internal part of cry11Ba (PstI/HpaI fragment from pJEG80.2) (□), and the 5′ end of cry11A (BamHI/SphI fragment from pPS401) ().

Inline graphic — Construction of the recombinant B. sphaericus strains by homologous recombination. The genes were introduced either upstream from the binary toxin (cry11A) or at the protease locus (cry11A, cry11Ba, and aphA3). E1, EcoRI. Probes used in Southern blot analysis were the 5′ end of the binary toxin (BamHI/BglII fragment from pHT560) (), the 5′ end of the protease gene (BamHI/SalI fragment from pPS408) (▪), the internal part of cry11Ba (PstI/HpaI fragment from pJEG80.2) (□), and the 5′ end of cry11A (BamHI/SphI fragment from pPS401) ().