Protein analysis of spore-crystal mixtures from wild-type and recombinant B. sphaericus strains producing the B. thuringiensis toxins. Spore-crystal suspensions (10 μg of protein) were subjected to SDS-PAGE (10% polyacrylamide) followed by staining with Coomassie blue. Lane 1, wild-type B. sphaericus 2297; lane 2, 2297pro::aphA3; lane 3, 2297bin::cry11A; lane 4, 2297bin::cry11A pro::cry11Ba; lane 5, 2297pro::cry11Ba; and lane 6, 2297pro::cry11A. Arrows indicate the positions of Cry11A (66 kDa) and Cry11Ba (80 kDa).