FIG. 4.

Detection (indicated by arrows) of Cry11A (A) and Cry11Ba (B) in various B. sphaericus 2297 strains. Spore-crystal mixtures (1 μg of protein) were subjected to SDS-PAGE (10% polyacrylamide) and the proteins were transferred onto a nitrocellulose membrane. The membrane was incubated with antisera raised against either total solubilized crystals from B. thuringiensis subsp. israelensis (A) or solubilized Cry11Ba inclusions (B). Lanes 1, wild-type B. sphaericus 2297; lanes 2, B. sphaericus 2297bin::cry11A pro::cry11Ba; lane A3, B. sphaericus 2297bin::cry11A; lane B3, B. sphaericus 2297pro::cry11Ba; and lane A4, B. sphaericus 2297pro::cry11A. Immunoreactive polypeptides were detected with a peroxidase-conjugated secondary antibody.