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. 1999 Jul;65(7):3027–3032. doi: 10.1128/aem.65.7.3027-3032.1999

FIG. 4.

FIG. 4

SDS-PAGE analysis of samples from each purification step. Lane 1, molecular mass standards; lane 2, inclusion body fraction after washing with double-distilled H2O; lane 3, supernatant after denaturation and centrifugation; lane 4, refolded leptin after dialysis; lane 5, sample after anion-exchange chromatography.