Table 1.
RNA Target probes used across the 12 rounds of ISHa
| Fluorophore label | Round 1 | Round 2 | Round 3 | Round 4 |
|---|---|---|---|---|
| AF488 | Leptin receptor B (catalog #402731) | NPY (catalog #313321) | ChAT (catalog #408731) | mCherry (catalog #431201) |
| Atto550 | Kappa opioid receptor (catalog #316111) | TRH (catalog #4368110 | Galanin (catalog #400961) | VGLUT2 (catalog #319171) |
| Atto647 | NPY receptor 1 (catalog #4270210) | Orexin (catalog #490461) | VGAT (catalog #319191) | NeuN (catalog #313311) |
aThree RNA targets were imaged in each round, alongside DAPI. The fluorophores were cleaved and then attached to the next round of targets across four rounds. NPY, Neuropeptide Y; TRH, thyrotropin releasing hormone.