Figure 5.

SDC3 siRNA#1 depletion in (A) SKOV3 and (B) CAOV3 cells results in reduced activation of Stat3. SKOV3 and CAOV3 cells were subjected to control or SDC3 siRNA#1 treatment, serum starved for 24 h and stimulated with 2 ng/mL of the cytokine Midkine (MDK) for the indicated timepoints. Cell lysates were subjected to Western blotting for the active, phosphorylated form of Stat3, total Stat3 and Gapdh as a loading control. SDC3 depletion reduces the constitutive activation of Stat3 in both cell lines. Representative blot from 3 experiments. Total Stat3 and Gapdh were detected on the same membrane after antibody stripping. (C) String analysis reveals direct functional interactions of SDC3 with SDC1 and Notch1 and indirect interactions with constituents of the stemness-associated Notch, Wnt and hedgehog pathways. Interaction analysis of SDC3 with SDC1, STAT3, the Notch pathway constituents NOTCH1 and HES1, the Wnt signaling constituents WNT5A and TCF7L1, the hedgehog pathway constituents GLI1–3, and the EMT-related markers E-cadherin (CDH1) and Snail (SNAI1) was performed using the STRING database (http://string-db.org/, accessed on 4 June 2021).