Table 2.
PCR primer sequences used in this study.
| mRNA | Sequence (Forward) | Sequence (Reverse) |
|---|---|---|
| ACTB | TCAAGATCATTGCTCCTCCTGAG | ACATCTGCTGGAAGGTGGACA |
| CDH1 | CAAAGCCCAGAATCCCCAAG | CACACCTGGAATTGGGCAAA |
| Gli1 | TTCCTACCAGAGTCCCAAGT | CCCTATGTGAAGCCCTATTT |
| Gli2 | GTCAGAGCCATCAAGACCGAGA | GCATCTCCACGCCACTGTCATT |
| Gli3 | TCAGCAAGTGGCTCCTATGGTC | GCTCTGTTGTCGGCTTAGGATC |
| Hes1 | GTGAAGCACCTCCGGAAC | CGTTCATGCACTCGCTGA |
| Hey2 | TTGCCCATGCCTAAACTAGTGC | TCTCACGTGCTTGATTTCAGCATA |
| Msi1 | AGCTTCCCTCTCCCTCATTCC | GGAATTCCAGGGTCCTGAGC |
| Msi2 | CCTAGTATGCTTGCCTCACAAACG | CGTCATCAGGGAGAAGCACAG |
| Notch1 | GGTGAGACCTGCCTGAATG | GTTGGGGTCCTGGCATC |
| PTCH1 | GGTGGCACAGTCAAGAACA | ACCAAGAGCGAGAAATGG |
| Robo1 | GCATCGCTGGAAGTAGCCATACT | CATGAAATGGTGGGCTCAGGAT |
| SDC3 | TGCCTCAGAAGAGTATCCTG | CTTGTCAGGCTTCTGGTATG |
| SFRP1 | GATGCAGGAGGCTCAGGTGAT | GCTGGCAACAGGTCAGAACG |
| SHH | GAAAGCAGAGAACTCGGTGG | GGAAAGTGAGGAAGTCGCTG |
| Snail | CGAGCCCAGGCAGCTATTTC | CCCGACAAGTGACAGCCATT |
| Src | ACCACCTTTGTGGCCCTCTATG | GCCACCAGTCTCCCTCTGTGTT |
| TCF7L1 | AAGGTGCCTGCCACTTCCTC | CCTGCCACTCTGGGATTGTG |
| TCF7L2 | AAACCAGCTGCCGCTTTTATG | GCAACATCAACATGCCTAGGTTTT |
| Twist | GCGGCCAGGTACATCGACTT | TGCAGCTTGCCATCTTGGAG |
| Vimentin | TCAGCATCACGATGACCTTGAA | CTGCAGAAAGGCACTTGAAAGC |
| Wnt 3A | AGGTCCCACAGCCCTGAGAT | TCCAGGAAAGCGGACCATTT |
| Wnt 5A | TCGTTAGCAGCATCAGTCCACA | GACCTGTGCCTTCGTGCCTA |
| ZEB1 | CAGCCCTGCAGTCCAAGAAC | TTGTCTTTCATCCTGATTTCCATTT |