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Details of primers used in this study.
| Target | ID | Primer Sequence (5′-3′) | PCR Size |
|---|---|---|---|
| SD+ Flag |
SDF | TACGTAGCGAGGAGTGACGATAAAGATGAAATTAAAGCAA | 161 bp |
| SDR | CTTATCGTCGTCATCCTTGTAATCAAGTCGACCATCAGCTTTAACTGTTG | ||
| S1 | S1F | GTCGACTTGATTACAAGGATGACGACGATAAGTGCATTGGTTAT | 1518 bp |
| S1R | GGGCCCCTAGTAAAAGAAACCAGGCAACTC |
Bold type indicates restriction enzyme recognition sites used for cloning.
