Figure 8.

VacV cytotoxicity against the S. oryzae (A) Mean larvae survival (±SE) of S. oryzae adults in response to VacV protein after nine days of treatment. (B) S. oryzae mean percent mortality (±SE) after four treatments of VacV sprayed on rice kernels. In comparison to each other and to the control, two VacV treatments (1.5, 2.0, and 3.0 mg) resulted in considerable mortality. However, there was no significant mortality with the 1.0 mg therapy. After every third day, up to a maximum of nine days, data was collected until the adults mated and laid the eggs. (C) After 35 days, VacV protein effectiveness against S. oryzae offspring. Purified VacV has a considerable effect on growing larvae, as shown in the graph. A maximum protein concentration of 3.0 mg resulted in a significant reduction in developing larvae. (D) In response to VacV, two generations of S. oryzae offspring were investigated. After 35 (first life cycle; F1) and 65 (second life cycle; F2) days, the effect of VacV on two generations (F1 and F2) of S. oryzae offspring was observed. In comparison to the control, VacV caused considerable mortality in the F1 generation; however, the F2 generation demonstrated less mortality in the adults. Nonetheless, when compared to the other therapies, the highest dosage (3.0 mg) resulted in considerable mortality.