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. 2022 May 6;14(5):1002. doi: 10.3390/pharmaceutics14051002

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of U87 exo, A549 exo, U87-Selu exo, and A549-Selu exo. (a) NTA results showed the mean and mode size of the exosomes (black: mean value, red: error). The loading of selumetinib changed the size distribution of the exosomes for both U87 and A549 derived exosomes. n = 3. (b) TEM images showed that all exosomes were round in shape. (c) Immunoblotting results revealed that U87-derived exosome-specific exosomal markers (CD proteins and TSG 101) were highly expressed in U87-derived exosomes, but not in A549-derived exosomes. A non-exosomal marker (Calnexin) was used as reference, n = 3.