Figure 4.

Viability of cells used for gene expression analysis. PMA-activated THP-1 wildtype (WT) cells were exposed to GNP-1, GNP-4, and GNP-6, and PMA-activated, ASC-deficient, and NLRP3-deficient cells were exposed to GNP-6. Cells were PMA-stimulated for 3 h, allowed to rest for 24 h, and exposed for 48 h, after which, cell viability was measured. Viability: percentage of untreated controls ± SD (N = 3). All exposure concentrations were 0.05 nM; this translates to the different weight concentrations indicated.