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Radiosynthesis and quality control of probe [18F]SF-AAN. (A) Radiosynthetic route of probe [18F]SF-AAN. [19F]SF-AAN (25 mM, 20 μL) was incubated in pyridazine-HCl buffer (350 μL) at 80 °C for 30 min. (B) HPLC characterization of [18F]SF-AAN before and after purification. (C) Stability assay of tracer [18F]SF-AAN in PBS at 37 °C (1, 2 and 4 h) and venous blood of nude mice at 30 min post injection.
