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. 2022 May 8;14(5):1013. doi: 10.3390/pharmaceutics14051013

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Protein-mediated shielding of Ag NP cytotoxicity. Affibody Z_HER2:342 cell labeling. (a): MTT assay results of the SKOV3-1ip and CHO cells viability (%) depending on the concentration of unmodified Ag NPs and BSA-modified Ag-BSA NPs in the medium (µg/mL). (b): Confocal microscopy of HER2-overexpressing SKOV3-1ip and HER2-negative CHO cells labeled with Z_HER2:342-FITC. (c): Flow cytometry histograms of SKOV3-1ip and CHO cells showing the cell interaction with Z_HER2:342-FITC. Green—cell autofluorescence, blue—cells labeled with affibody.