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. 2022 May 8;14(5):1013. doi: 10.3390/pharmaceutics14051013

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Imaging modalities of Ag-PEG-HER2 nanoparticles. (a): Visualization of the internalization of Ag-PEG-Z_HER2:342 NPs in SKOV3-1ip cells after incubation for 30 (left panels) and 120 (right panels) min using two-channel confocal microscopy. Cell nuclei are stained with Hoechst33342 (blue), and NPs are labeled with DyLight650 (red). (b): Flow cytometry histograms of SKOV3-1ip, BT474, SK-BR-3, A549, MCF-7, and CHO cells showing the cell interaction with Trastazumab-DyLight650 and Ag-PEG-HER2*DyLight650. Gray—cell autofluorescence; blue—cells labeled with Trastuzumab; and violet—cells labeled with Ag-PEG-HER2*DyLight650 nanoparticles.