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. 2022 May 21;23(10):5774. doi: 10.3390/ijms23105774

Figure 3.

Figure 3

The effect of 4PYR on the invasive potential of 4T1 cells is blocked by CD73 inhibition and mediated through adenosine receptors (AR). (A) Transwell invasion assay of 4T1 cells treated with 4PYR (100 μM), CD73 inhibitor, AOPCP (200 μM; inh. CD73), or their combination, relative to vehicle-treated control. (B) Transwell invasion assay of 4T1 cells treated with 4PYR (100 μM), specific AR agonists (ag. AR A3, IB-MECA for A3AR; ag. AR A2A, CGS-21680 for A2AAR; 10 μM of each agonist), or their combination in the presence of CD73 inhibitor (to inhibit endogenous eAdo generation), relative to vehicle-treated control (100%; the bar is not shown). Cells that invaded through Matrigel in the Transwell invasion chamber after 24 h were stained with Mayer’s Hematoxylin and counted under the microscope. The graph represents the mean ± SEM from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.