TABLE 3.
Results of culture on SKDM agar, nested RT PCR of RNA extracts, and PCR of DNA extracts from kidney tissue from representative live Atlantic salmon challenged with R. salmoninarum by cohabitation
| Fish no. | CFU g−1 (wet wt) of kidneya | Nested RT PCR assayb | PCR assayc |
|---|---|---|---|
| 1G | >5 × 107 | + | + |
| 2J | >5 × 107 | + | + |
| 3H | 2.4 × 106 | + | NPd |
| 3J | 1.6 × 106 | + | NP |
| 3G | 8.5 × 105 | + | NP |
| 3D | 3.4 × 105 | + | NP |
| 2D | 2.0 × 105 | + | NP |
| 3I | 3.5 × 104 | + | NP |
| 2B | 3.0 × 104 | − | + |
| 3A | 2.1 × 104 | + | + |
| 1I | 2.1 × 104 | + | + |
| 2F | 1.2 × 104 | + (weak) | + |
| 3B | 9.2 × 103 | + (weak) | NP |
| 3C | 1.0 × 103 | − | NP |
| 1C | NDe | + (weak) | NP |
| 2C | ND | + (weak) | + |
| 1D | ND | − | + |
| 2A | ND | − | + |
| 2E | ND | − | + |
| 2H | ND | − | + |
| 1B | ND | − | − |
| 2G | ND | − | − |
a
After 6 weeks incubation at 15°C on SKDM agar (2).
b
Detection of nested RT PCR amplified product on silver-stained polyacrylamide gels.
c
Detection of PCR amplified product on silver-stained polyacrylamide gels.
d
NP, not performed.
e
ND, not detected.