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. 2022 May 28;23:404. doi: 10.1186/s12864-022-08631-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Expression of AIRE protein in mTEC 3.10 cell line as detected by western-blot. The mTEC cells were transfected (or not) with miR-155-5p mimic, causing significant decrease in the levels of this protein. A western-blot detection of AIRE after 24 h of transfection. The GAPDH protein was used as housekeeping and to normalize data. The lanes/bands showed were cropped from the blot displayed in the Supp. Figure 1. B Bar graph resulted from quantification of western-blot bands show the effect of miR-155-5p mimic transfection on AIRE expression. The data are presented as the means and standard error of mean (SEM) from three independent determinations. Difference between groups was analyzed by Student t-test, comparing control (not transfected) versus transfected cells (**P < 0.01)