Table 2.
Neutralization synergy analysis of plant-made CB6 with other anti-SARS-CoV-2 RBD neutralizing mAbs. FFA experiments were performed for each individual nAb as well as nAb combinations with each nAb at concentrations that correspond to its IC20, IC25, and IC50. Neutralization data from two independent experiments were analyzed using the HSA and Loewe models (SynergyFinder.org). Predicted neutralization values represent the percent neutralization of nAb combinations, where there is no synergistic interaction between different nAbs in the cocktail at the indicated IC value. These predicated neutralization values were calculated from the actual neutralization data of each individual nAb in the cocktail, assuming there is no interaction between the nAbs in the cocktail.
| Cocktail Combination & Concentration | Observed Percent Neutralization | HSA Predicted Neutralization | Loewe Predicted Neutralization |
|---|---|---|---|
| CB6 + 3C4 (IC20) | 51.66% | 33.97% | 33.94% |
| CB6 + 11D7 (IC20) | 63.18% | 33.97% | 36.06% |
| CB6 + 3C4 +11D7 (IC20) | 60.84% | 33.7% | 36.08% |
| CB6 + 3C4 (IC25) | 58.65% | 33.15% | 44.54% |
| CB6 + 11D7 (IC25) | 78.2% | 33.15% | 42.42% |
| CB6 + 3C4 + 11D7 (IC25) | 77.87% | 34.1% | 41.31% |
| CB6 + 3C4 (IC50) | 67.09% | 57.21% | 61.5% |
| CB6 + 11D7 (IC50) | 87.04% | 57.21% | 57.26% |
| CB6 + 3C4 + 11D7 (IC50) | 88.49% | 47.48% | 65.59% |