Figure 4.

Comparative analysis of the production of some proteins in cells of L. capsici strains. (a) SDS-PAGE: Lanes correspond to samples of the culture liquid of L. capsici strains (Supplementary Figure S6). The gel was stained with imidasole–ZnCl₂ solution. (b) SDS-PAGE: Lanes correspond to samples of L. capsici strain cells and purified Blp of L. capsici VKM B-2533^T (Supplementary Figures S7 and S8). The gel was stained with a solution of Coomassie Brilliant Blue R-250. (c) Peak area of protein L1 at purification on a Hiload 16/60 column (Superdex 75). The mean values were obtained in two independent experiments, each with two technical replicates. The two groups were compared using a two-sided Mann–Whitney U-test (p = 0.0286). (d) Peak area of a mixture of serine proteases at the purification on a Hiload 16/60 column (Superdex 75). The mean values were obtained in two independent experiments, each with two technical replicates. The two groups were compared using a two-sided Mann–Whitney U-test (p = 0.0286). (e) Relative level of expression of the alpA gene in L. capsici P_T5-blp. The boxplots show the median and IQR. The values were obtained in four independent experiments, each with two technical replicates. The two groups were compared using an unpaired two-tailed Student’s t-test, t = 10.07, df = 18, * p = 0.0286.