Figure 6.
Assessment of the possibility of using the developed expression system for other L. capsici proteins. (a) SDS-PAGE: Lanes correspond to samples of culture liquid of L. capsici strains and purified serine protease (Serp) of L. capsici VKM B-2533T (Supplementary Figures S10–S12). The gel was stained with imidasole–ZnCl2 solution. (b) Relative level of expression of the serp gene of L. capsici serp PT5 as compared with wild-type L. capsici. The boxplots show the median and IQR. The values were obtained in four independent experiments, each with two technical replicates. The two groups were compared using an unpaired two-tailed Student’s t-test, t = 37.41, df = 14.