Table 3.
Strains and plasmids used in the work.
Strains and Plasmids | Characteristics | Ref. |
---|---|---|
L. capsici VKM B-2533T | Wild type | [33] |
L. enzymogenes VKM B-2235T | Wild type | [34] |
L. capsici PGroEL-gfp | Strain L. capsici VKM B-2533T with plasmid PBBR1-MCS5 PGroEL-gfp |
This work |
L. capsici PGroEL(A)-gfp | Strain L. capsici VKM B-2533T with plasmid PBBR1-MCS5 PGroEL(A)-gfp | This work |
L. capsici PT5-gfp | Strain L. capsici VKM B-2533T with plasmid PBBR1-MCS5 PT5-gfp | This work |
L. capsici PGroEL(A)-blp | Strain L. capsici VKM B-2533T with plasmid PBBR1-MCS5 PGroEL(A)-blp | This work |
L. capsici PT5-blp | Strain L. capsici VKM B-2533T with plasmid PBBR1-MCS5 PT5-blp | This work |
L. capsici PT5-serp | Strain L. capsici VKM B-2533T with plasmid PBBR1-MCS5 PT5-serp |
This work |
E. coli XL1–Blue | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 lac/[F′::Tn10 proAB + lacIq lacZΔM15 traD36] | [35] |
pBBR1-MCS5 * | Vector with PLac-promoter, GmR | [36] |
pTurboGFP-B | Template for amplification of fragment T5 promoter—gfp—terminator lambda t0 | [37] |
pEX18 | Template for amplification of terminators T1 and T2 | [38] |
PBBR1-MCS5 PGroEL-gfp | Plasmid pBBR1-MCS5 with gfp gene under the regulation of GroEL promoter | This work |
PBBR1-MCS5 PGroEL(A)-gfp | Plasmid pBBR1-MCS5 with gfp gene under the regulation of GroEL(A) promoter | This work |
PBBR1-MCS5 PT5-gfp | Plasmid pBBR1-MCS5 with gfp gene under the regulation of T5 promoter | This work |
PBBR1-MCS5 PGroEL(A)-blp | Plasmid pBBR1-MCS5 with blp gene under the regulation of GroEL(A) promoter | This work |
PBBR1-MCS5 PT5-blp | Plasmid pBBR1-MCS5 with blp gene under the regulation of T5 promoter | This work |
PBBR1-MCS5 PT5-serp | Plasmid pBBR1-MCS5 with serp gene under the regulation of T5 promoter | This work |
* The plasmid was a kind gift of Dr. Yangyang Zhao (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety—State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China).