Table 2.
Genes | Initial Period | Cycles, Denaturation, Annealing and Elongation | Final Extension | References |
---|---|---|---|---|
ITS, LSU, SSU, tef1 | 94 °C for 3 min | 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 50 s, elongation at 72 °C for 1 min | 72 °C for 10 min | [17] |
rpb2 | 95 °C for 5 min | 40 cycles of denaturation at 95 °C for 1 min, annealing at 52 °C for 2 min, elongation at 72 °C for 90 s | 72 °C for 10 min | [17] |