Skip to main content
. 2022 May 20;8(5):532. doi: 10.3390/jof8050532

Table 2.

ITS, SSU, LSU, tef1, and rpb2 loci PCR conditions.

Genes Initial Period Cycles, Denaturation, Annealing and Elongation Final Extension References
ITS, LSU, SSU, tef1 94 °C for 3 min 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 50 s, elongation at 72 °C for 1 min 72 °C for 10 min [17]
rpb2 95 °C for 5 min 40 cycles of denaturation at 95 °C for 1 min, annealing at 52 °C for 2 min, elongation at 72 °C for 90 s 72 °C for 10 min [17]