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. 2022 May 15;11(10):2785. doi: 10.3390/jcm11102785

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Sorafenib reduces migration of CAF and CAF co-cultivated CCA cells. HuCCT-1 and TFK-1 cells were cultured either alone or together with CAF or SC in 4 chamber ibidi slides. Mono-cultured CAF and SC were cultured in parallel as control. Additionally, cells were either left untreated or treated with 1 µM Sorafenib. (A) Gap closure of mono- and co-cultures with HuCCT-1 cells was analyzed after 18 h and those with TFK-1 cells after (B) 18 h and (C) 68 h. Data are presented as % gap closure normalized to t = 0 h and as mean and standard deviation of 3 independent experiments. Statistical significances are indicated in the figure.