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. 2022 May 23;10(5):825. doi: 10.3390/vaccines10050825

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The frequency of spot-forming cells expressing IFN-γ or IL-2 was determined by ELISPOT. The magnitude of the IFN-γ-secretion response produced by stimulation of splenic lymphocytes in normal mice and Mtb latently infected mice with Ag85b (A), ESAT6 (B), and CFP10 (C) peptides. The secretion of IL-2 cells stimulated by Ag85b (D), ESAT6 (E), and CFP10 (F) peptides was detected in the splenocytes of the two mouse models. */** The infection-CT-AEC/BC02 group was compared with the infection group. ^#/^## The infection-CT-AEC/BC02 group was compared with the infection-CT-BC02 group. Statistical significance was set at */^# p < 0.05 or **/^## p < 0.01 (n = 5).