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. 2022 May 19;23(10):5690. doi: 10.3390/ijms23105690

Figure 9.

Figure 9

Left: Plots of the natural logarithm of Kb vs. pressure (in bar) for the complex formation between berberine and 22AG in the presence of 100 mM NaCl (black squares) and 100 mM KCl (red circles). The pressure dependence of ln(Kb) for the complex formation between berberine and RG-1 is also shown in the same graph: in the presence of 100 mM NaCl (blue triangles) and in the presence of 100 mM KCl (reversed green triangles). The evaluation of the reaction volume, ΔVr, was determined from the slope of the plots. Right: Reaction volumes, ΔVr, for the complex formation between berberine and the G4Qs (22AG and RG-1) for the reported solution conditions (30 mM Tris-HCl, pH 7.4, with the addition of 100 mM NaCl or 100 mM KCl) at the temperature of 25 °C.