Table 2.
Binding constants, Kb, and stoichiometries for the complex formation between berberine and G4Qs (22AG and RG-1) for the indicated solution conditions and at pressures of 1, 500, 1000, 1500, and 1900 bar (T = 25 °C).
| Berb/22AG NaCl (100 mM) | Berb/22AG KCl (100 mM) | ||||
| p/bar | Kb/M−1 ∙ 105 | 1 Berb:G4Q | p/bar | Kb/M−1 ∙ 105 | 1 Berb:G4Q |
| 1 | 0.37 ± 0.09 | 2:1 | 1 | 5.9 ± 1.0 | 2:1 |
| 500 | 0.39 ± 0.07 | 2:1 | 500 | 6.6 ± 0.9 | 2:1 |
| 1000 | 0.44 ± 0.06 | 2:1 | 1000 | 6.9 ± 0.7 | 2:1 |
| 1500 | 0.43 ± 0.09 | 3:1 | 1500 | 7.1 ± 0.4 | 2:1 |
| 1900 | 0.50 ± 0.09 | 3:1 | 1900 | 7.3 ± 0.5 | 2:1 |
| Berb/RG-1 NaCl (100 mM) | Berb/RG-1 KCl (100 mM) | ||||
| p/bar | Kb/M−1 ∙ 105 | 1 Berb:G4Q | p/bar | Kb/M−1 ∙ 105 | 1 Berb:G4Q |
| 1 | 0.28 ± 0.03 | 2:1 | 1 | 0.14 ± 0.03 | 2:1 |
| 500 | 0.38 ± 0.05 | 2:1 | 500 | 0.17 ± 0.03 | 2:1 |
| 1000 | 0.49 ± 0.06 | 2:1 | 1000 | 0.23 ± 0.02 | 2:1 |
| 1500 | 0.63 ± 0.07 | 2:1 | 1500 | 0.31 ± 0.01 | 2:1 |
| 1900 | 0.77 ± 0.09 | 2:1 | 1900 | 0.40 ± 0.06 | 2:1 |
1 Stoichiometry of the complexes.