Figure 1.

CD47-binding peptide inhibits leukocyte adhesion to retinal vessels in IRBP-induced chronic uveitis in mice. WT mice were immunized s.c. with IRBP (200 µg) in complete Freund’s Adjuvant and treated once daily with topical application of 4N1K or 4NGG (5 µg per eye). (a) Whole mounts of retinas were harvested 7 days post-immunization from rhodamine-ConA perfused mice and imaged to visualize firmly adherent leukocytes (arrows) in retinal vessels. The inset shows a digital magnification of an area. Quantitative analysis of adherent leukocytes is presented in the bar graph. Data are presented as the mean adherent leukocytes per mm² ± SEM. (b) Clinical course of immunized and treated mice was followed for 30 days post-immunization by weekly fundus exam and scoring, as described in Materials and Methods. Results are expressed as the mean scores ± SEM with representative fundus images. n = 5 per group per experiment. * p < 0.05, **** p < 0.0001. Scale bar = 100 µM.