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. 2022 May 20;23(10):5705. doi: 10.3390/ijms23105705

Figure 4.

Figure 4

IL-17 and TNF-α both increase expression of VCAM-1 on vascular endothelial cells but differentially modulate endogenous TSP-1 expression. Primary cultures of murine vascular endothelial cells were treated with TNF-α (10 ng/mL) or IL-17 (10 ng/mL) for 24 h. (a) Endothelial cells were immunostained with anti-VCAM-1 (red), as described in Materials and Methods (200× magnification), nuclei stained with DAPI (blue) (b) Intracellular TSP-1 in permeabilized endothelial cells was detected by flow cytometry. Percentage indicates the proportion of cells positively stained for TSP-1, (c) Real-time PCR was performed on RNA isolated from endothelial cells to detect TSP-1 message. Results are expressed as the mean ± SEM (n = 3 per experiment; * p < 0.05).