Figure 1.

Measurement of cytoskeleton structures and focal adhesions of MC3T3-E1 cells cultured under different conditions. MC3T3-E1 cells were cultured in chamber slides under 1 g (A,D), μg (B,E) and μg + CNF1 (C,F). The cells on slides were stained with (A–C) FITC-phalloidin (green) and (D–F) anti-paxillin antibody (green). Slides were then covered with cover slips using Prolong Gold Antifade Reagent with DAPI (blue) and analyzed for microfilament (green) and focal adhesions (green), respectively, by fluorescence microscopy using 40× objectives (formation of cellular focal adhesions, white arrows). The average numbers of cellular focal adhesions per 40× field were measured by using ImageJ software. Data represent the mean ± SD. * p < 0.05 versus different groups by using Student t test. One representative experiment of two is shown.