Figure 3.

Actin immunofluorescence studies. MDA-MB-231 cells were exposed to compound 1 (used at its IC₅₀ value), with 0.1 μM Latrunculin A (LA) or with a vehicle (CTRL) for 24 h. Then the cells were further processed, observed and imaged under the inverted fluorescence microscope at 40x magnification. CTRL cells showed a regular arrangement and organization of the actin cytoskeleton. MDA-MB-231 cells exposed to LA and, as well as those treated with compound 1, exerted an irregular arrangement and organization of the actin system. Panels (A): nuclear stain with DAPI (λ_ex/λ_em = 350/460 nm); Panels (B): β-actin (Alexa Fluor^® 568; λ_ex/λ_em = 644/665 nm); Panels (C): show a merge. Representative fields are shown.