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Mitochondria staining and cytochrome c detection in MDA-MB-231 cells. In the CTRL cells as well as in the cells treated with compound 1 at its IC50 value for 24 h, cytochrome c is localized within mitochondria. Panels (A), DAPI λex/em = 350/460 nm; Panels (B), Alexa Fluor CF 488 λex/em = 490/515 nm; Panels (C), MitoTracker Deep Red FM probe λex/em = 644/665 nm; Panels (D), overlay channels. Images were acquired at 40× magnification and representative fields are shown.
