Figure 13.

NF-κB activation in MDA-MB-231 and MCF-7 cells after treatment with compounds 1 and 6, respectively. In the vehicle-treated cells, NF-κB is localized within the cytoplasm and nucleus, while the treatment of the breast cancer cells with compounds 1 and 6 for 24 h (used at their IC₅₀ values) reduced the NF-κB presence in the nucleus. Panels A, nuclear stain with DAPI (λ_ex/em = 350/460 nm); Panels B, Alexa Fluor CF 488 (λ_ex/em = 490/515 nm); Panels C, overlay channels. Images were acquired at 40× magnification and representative fields are shown.