Table 1.
Comparison of different NGS platforms: Illumina MiSeq, Illumina HiSeq 2000, Ion Torrent PGM, PacBio SMRT and Oxford Nanopore MinION.
| Illumina MiSeq | Illumina HiSeq 2000 | Ion Torrent PGM | PacBio SMRT | Oxford Nanopore MinION | |
|---|---|---|---|---|---|
| Read Length | Up to 150 bases | Up to 150 bases | ~200 bases | Average 1500 bases | 13–20 kb |
| Paired-End | Yes | Yes | Yes | No | No |
| Reported Accuracy | Mostly >Q30 | Mostly >Q30 | Mostly Q20 | <Q10 | Mostly Q50 |
| Observed Raw Error Rate | 0.80% | 0.26% | 1.71% | 12.86% | 10.50% |
| Insert Size | Up to 700 bases | Up to 700 bases | Up to 250 bases | Up to 10 kb | Average of 331 bases |
| Run Time | 27 h | 11 days | 2 h | 2 h | 72 h |