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Plasmid maps. Abbreviations: NG2 rep, open reading frame from the NG2 replicon permitting plasmid replication in both E. coli and C. glutamicum; Ptrc, trc promoter from pTrc99a; lacI, open reading frame encoding the lac repressor from pTrc99a; KmR, kanamycin resistance gene from pEP2. NsiI/PstI 2719, NsiI/PstI 1532, and NsiI/PstI 2916 indicate the positions of hybrid NsiI and PstI sites resulting from ligation.
