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. 2022 Apr 27;15(5):540. doi: 10.3390/ph15050540

Figure 6.

Figure 6

MTT assay and inhibition of SARS-CoV-2 by D-CDP1 and D-CDP7. (A): MTT assay of D-CDP1 and D-CDP7 on Vero CCL-81 cells. MTT assay was used to evaluate the cytotoxicity of the two D-peptides. Different concentrations of up to 98 µM were used to treat the Vero cells for 2 days. Data shown are the means ± SD from three independent measurements (n = 3). (B): Inhibition of SARS-CoV-2 by D-CDP1 and D-CDP7. Vero cells were treated with 50 µM of either D-CDP1 or D-CDP7, and after an hour of incubation, the cells were infected with SARS-CoV-2 using a multiplicity of infection (MOI) of 0.05. Untreated cells were used as control. Viral RNA in the supernatant was analysed in-house. The statistical significance of the mean values’ differences was assessed with a standard two-tailed unpaired t-test (demonstrated by asterisks). The graph shows individual data points with mean ± SD (n = 6).