Figure 1.

Gene array analysis demonstrated that EMT genes were regulated in U-87 MG cells treated with TGF-β1. Total RNA was isolated from U-87 MG cells treated, or not, with 10 ng/mL TGF-β1 for 24 h, then cDNA was synthesized. The Human EMT signaling pathways RT² Profiler PCR Arrays served to identify EMT genes that were regulated following TGF-β1 treatment as described in the Materials and Methods section. (A) Graphic representation of the array showing gene expression levels. The values of upregulated genes are colored by red points, and those of downregulated genes are colored by green points. Data are delimited by external lines. Values higher than +2.00-fold and lower than −2.00-fold induction by TGF-β1 were considered significant. (B) Representative values of changes in EMT gene expression by TGF-β1 treatment in U-87 MG cells.