Figure 5.
H295R-PM-Ptc+ cells are more tumorigenic and metastatic than parental ACC cells in ovo. (A) Schematic representation of the study. On day 9 of egg development (E9) 3 × 106 H295R-PM-Ptc+ and H295R cells were injected in the chorioallantoic membrane (CAM) from two groups of 20 eggs. At E18 tumors from CAM and embryos were collected. (B) H295R-PM-Ptc tumors collected on E18 were bigger than H295R tumors (n = 15 for H295R graft and n = 21 for H295R-PM-Ptc+; ****: p-value < 0.00005). (C) Metastasis in liver was addressed using human Alu sequences by real time qPCR. H295R-PM-Ptc+ xenografted cells form more metastases in the embryo liver compared to H295R xenografted cells (mean ± SEM n = 9, *: p-value < 0.05 (p = 0.03)).